Theoretical and empirical number of deconvolutable cells recovered across various nuclei loading inputs for molecular and cellular hashing strategies. The simulated cell recovery for either molecular hashing at different numbers of Tn5 barcodes (black and gray lines) or cellular hashing (blue line) strategies were compared with the observed cell recovery obtained from the txci-ATAC (indexed with 96 Tn5 barcodes, red dots), dsciATAC (indexed with 48 Tn5 barcodes, slate blue dots), and SNuBar (blue dots) datasets. For txci-ATAC and dsciATAC, the data were processed using the same pipeline, and the cell threshold was determined using K -means clustering. For SNuBar, the recovery values were taken directly from the original paper. The lower panel zooms in on the range highlighted by the dashed box in the upper panel.
