Spectra for a solution containing UppP (3.5 M) and 20 M C55-PP incubated in the presence of 100 M bacitracin (top) and 100 M teixobactin (lower panel) for 30 min. Only a small amount of enzyme product complex is detected in the presence of both inhibitors, reflecting that both inhibitors sequester the lipid substrate C55-PP from the enzyme. Them/zvalues for antibiotics and their complexes with the substrate are labeled. Shown on the right are the chemical structures of bacitracin and teixobactin.
