Spectra of 3.5 M S26A UppP titrated with 10 M C55-PP, 10 M DGPP, and 20 M C15-PP. C55-PP and DGPP bind more favorably to the enzyme than C15-PP. (Inserts) mean relative abundance of ligand-free and ligand-bound S26A UppP in the spectra. Error bars are standard deviations of three replicate measurements.
