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Two-hybrid analysis in E. coli BTH101 cells expressing T25 and T18 fusions of CarHMx(HMx), CarHTt(HTt) or, as positive control under all tested conditions, CarAMx(AMx). Streaks of cells on LB-Kan-Amp agar plates containing X-Gal and IPTG without or with AdoCbl or AdoRhbl grown in the dark or in white light. Blue streak indicates self-interaction.

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