Protein Structure from Scientific Research

Left: Close-up of the dark CarHTt tetramer AdoCbl-binding pocket (PDB ID 5C8D) highlighting Trp131, Glu141, and His142 (gold) of the Wx9EH motif capping the Ado group (cyan) of AdoCbl (gray with cobalt as magenta sphere), and the lower axial cobalt ligand His177 (green). Right: EMSA binding of the indicated CarHTt mutants to the 130-bp DNA probe in the dark with 5-fold molar excess of AdoCbl or AdoRhbl. Uncropped gel is shown in Figure S14B.

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