Line Plot from Scientific Research

Open access visualization of Line Plot, Ferroxidase Centre, Iron Release, Bfr, Absorbance
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Increase in 563 nm absorbance as a function of time following the addition of 100 M FMN to solutions containing ferrozine and iron-loaded Bfr reconstituted with 9 hemes per cage in which the ferroxidase centres had been inactivated by Zn2+binding (black) and an equivalent sample reconstituted with 8 Zn protoporphyrin IX per cage in place of heme (blue). Red trace depicts the response of a sample reconstituted with 8 Zn protoporphyrin IX per cage in which the ferroxidase centres remained active, and the broken black trace that of a sample reconstituted with 9 hemes per cage in which ferroxidase centres remained active.

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