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FMN-driven iron release from Bfr. Increase in 563 nm absorbance as a function of time following the addition of 100 μmFMN to solutions containing ferrozine and iron-loaded Bfr reconstituted with 9 hemes per cage (black), reconstituted with 8 Zn Protoporphyrin IX per cage (red) or with no reconstitution of vacant heme sites (blue).
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