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IB of EsxA or EsxB in pellets and supernatant from H37Rv, H37RvΔEsxB, or H37Rv(ΔEsxB + EsxB WT/mutants ) strains. Results in b are representative images from one of three independent experiments. All of the bar graphs in this figure reflect the mean ± SEM from three independent biological experiments. Two-tailed unpaired Student’s t -test were used.

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