Nox2 mRNA stability analysis in macrophages after actinomycin D treatment. Mouse peritoneal macrophages were infected with H37Rv, H37Rv:ΔEsxB, H37Rv(ΔEsxB + GFP), and H37Rv(ΔEsxB + EsxB) (MOI = 5) for 4 h and treated with actinomycin D (5 μg/mL). Cells were harvested at the indicated timepoints. Expression levels were normalized to 0 h and Gapdh was used as a reference gene. Results in d are representative images from one of three independent experiments. All of the results (except d ) reflect the mean ± SEM from three independent biological experiments. Two-tailed unpaired Student’s t -test were used in a – c and e – g .
