Off-target effects of TnpB ( j ) and TnpB379 ( k ) and Cas9 ( l ) targeting the CCR5 gene respectively. The underlined sequences represent the on-target or predicted off-target sites. The mismatches were labeled in red and lower case. The DNA bulges were labeled in green and lower case. Each point represents the editing efficiency of independent biological replicates by deep sequencing analysis. Data are presented as means SD, n = 3 independent biological replicates.
