The phylogenetic tree was generated using the phylogeny.fr web service35and a multiple sequence alignment generated using Clustal Omega.36Genebank codes for sequence alignment: BVDV-1:NP_040937, BVDV-2: AMO65207, BVDV-3:YP_002967452, BDV:NP_620062, CSFV:NP_075354, APPV:YP_009268709, NRPV:YP_009109567, Aydin:YP_006860588, Giraffe:NP_620053, Pronghorn:YP_009026415, Bungowannah:YP_008992092, Linda:YP_009407716, Bat: AYV99177, Rodent: ATP66856, Phocoena: QFQ60724. APPV and NRPV E2 proteins described in this paper are colored in blue. The scale bar represents the number of substitutions per sites.