Quantification of TBR1+, CTIP2+, and NeuN+in d25 CYFIP1-GoF cultures after 10 days of exposure to increasing dose of 24S,25-EC or ethanol control. The red dashed line indicates the baseline (untreated) level in the isogenic control line, n = 3. Data shown are mean SEM of the number of biological replicates indicated in specific panels. Groups were compared by Student’s t test between the indicated genotype and respective control per time point in (A) and one-way ANOVA followed by Tukey’s correction for (D) and (F)–(H) (*p < 0.05;**p < 0.01;***p < 0.001). Nuclei were counterstained with DAPI (blue). Scale bars: 50 m.
