C6 mutants F72R and F75R restrict IRF3 activation less efficiently than WT C6. An ISG56.1-Luc reporter gene assay was performed with the indicated levels of TAP-tagged C6, C6 F72R, or F75R. Empty vector (EV) and TAP-tagged N1 were included as negative controls. The cells were stimulated with SeV for 16 h and then collected for measurement of firefly andRenillaLuc activity. Immunoblots below the graph show expression of TAP-tagged proteins and Tub.
