Expression of HDAC5 in U2OS cells restricts CPXV-BR, CMLV, and CPXV-E replication. U2OS.TetR.EYFP or U2OS.TetR.HDAC5-FLAG cells were mock-induced or induced with dox for 18 h and then infected with CPXV-BR, CMLV, and CPXV-E at 0.01 PFUs per cell for 2 days (CPXV-BR) or 3 days (CMLV and CPXV-E). The culture medium and infected cells were collected, and virus titer was quantified by plaque assay on BSC-1 cells. Data are presented as mean ± SEM, n ≥ 3 independent experiments.*p < 0.01,***p < 0.001,****p < 0.0001.