Expression of HDAC5, but not HDAC4, enhances TRIF-mediated IRF3 activation in parental HEK293T cells and HDAC5-/- cells. H5KO3 cells were transfected overnight with plasmids expressing ISG56.1-Luc, TK-Renilla, TAP-TRIFΔRIP, and HDAC4-FLAG. The HDAC4-FLAG-expressing plasmids were transfected at 0, 10, 50, or 100 ng per well. The relative ISG56.1-Luc reporter expression was analyzed as in Figure 1. Immunoblots for FLAG-tagged proteins and Tub are shown beneath the reporter gene assays. Data are presented as mean ± SEM, n ≥ 3 independent experiments.*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
