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Pax1gene expression levels from E12.5 mouse tail as determined by RT-qPCR. Each value represents the ratio ofPax1gene expression to that ofβ-Actin, and values are mean ± standard deviation. The expression value of wild type (WT) females was arbitrarily set at 1.0. Each dot represents one embryo, and statistical differences were determined using unpaired t test (****p < 0.001,***p < 0.005,**p < 0.01, ns, not significant).
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