Fluorescent staining of blastocysts for SOX2 and RFP, with SOX2 as an ICM marker and nuclear-localized RFP as a lineage tracer. Rfp+NC represents blastocysts developed after injecting RFP and NC into a single blastomere at the 2-cell stage. Rfp+SI1-1 and Rfp+SI1-2 depict blastocysts developed after injecting RFP and SI1, differing in the number of daughter cells developed from the injected blastomere compared to the uninjected one. Rfp+Nr5a2 indicates blastocysts developed after injecting RFP and Nr5a2. Scale bar: 50 m.
