Step-by-step identification of cross-neutralizing mAbs. Left: neutralization IC50 values of 87 supernatants against virus BG505.N611Q. A gray line indicates 10 supernatants with the lowest neutralizing IC50 values. Red circles indicate 9 supernatants with mAbs recovered. Center: neutralization IC50 values against autologous wild-type BG505 by 9 selected mAbs. Red circles indicate 6 mAbs with neutralizing IC50 values below 10 ug/mL (gray line). Right: neutralization IC50 values against a panel of 9 multiclade heterologous wild-type HIV-1 strains by 6 selected mAbs and the reference mAb DFPH-a.01. A red bar indicates median value.
