ECmockor ECRRas38Vwith and without Akt3 silencing (green) were cocultured with unlabeled parental ECs at a 1:3 ratio. VE-cadherin and Ki-67 were stained and imaged 48 h later. Ki-67+cells in the transduced EC population (green) and those in the parental (neighboring) EC population were quantified. Data are represented as the mean +/- SEM in violin plots.*p < 0.05,**p < 0.01,***p < 0.001; n.s., not significant. N = 4 wells, multiple pictures were analyzed for each well.
