ECmockor ECRRas38Vwith or without Jagged1 silencing were cocultured with parental ECs at a 1:3 ratio. Confluent monolayers of various coculture combinations were examined for endothelial permeability by dextran leakage. Single culture of ECRRas38Vwas included for comparison with the cocultures. N = 3. Data are represented as the mean SEM.