ECRRas38V(green) were cocultured with UNC5b-silenced or control ECs (unlabeled). The coculture was then stained for VE-cadherin (magenta). Top: white arrows indicate a strong accumulation of VE-cadherin at the cell membrane of control ECs adjacent to ECRRas38V. Bottom: yellow arrows indicate the loss of VE-cadherin at the cell-cell junctions in neighboring ECs in which UNC5B was silenced. Representative images of three independent experiments are shown. Scale bars, 10 m.
