Ki-67 staining of proliferating ECs in the coculture system. Green-labeled mock- (ECmock) or R-Ras38V-transduced ECs (ECRRas38V) were transfected with Jag1(siJag1) or control siRNA for 15 h and then cocultured with non-transduced unlabeled parental ECs (EC) for an additional 48 h. DAPI was used for nuclear staining (blue).