Vero E6-TMPRSS2 cells were infected with WT or N-F17A mutant SARS-CoV-2 (MOI 0.01). Cells were fixed for staining with the indicated antibodies at 24 h post infection (hpi). Yellow and white arrowheads indicate infected cells at early and late stages of infection, respectively, distinguished by the expression levels of viral N proteins. Scale bars, 20 m.