Gene expression analysis of ADH and PCO1 at ZT4, 16, 20, and 24 in 8-day-old Arabidopsis Col-0 shoots under LD, where the night was kept at normoxia, hypoxia, or hyperoxia. Dotted lines separate different time points. The mRNA levels of genes were measured by RTqPCR, and the data on the y-axis are expressed relative to ZT4 (ZT4 = 1). To calculate statistical significance, the KruskalWallis test followed by the post-hoc Wilcoxon test was carried out, and groups with significant differences (P< 0.05) are indicated with different letters. Boxplots show the center line as the median; the box extends from the 25th to 75th percentiles; whiskers represent 1.5 interquartile range; points outside the whiskers are outliers (n= 5 biological replicates).
