Gene expression analysis of ADH and PGB1 in the shoot of the seedling at 6, 8, and 10 DAG under LD. The mRNA levels of genes were measured by RTqPCR, and the data on the y-axis are expressed relative to ZT4 at 6 DAG (ZT4 at 6 DAG = 1). To calculate statistical significance, the KruskalWallis test followed by post-hoc Wilcoxon test was carried out, and groups with significant differences (P< 0.05) are indicated with different letters (number of replicates is shown in the figure). Boxplots center line, median. The box extends from the 25th to 75th percentiles; whiskers, 1.5 interquartile range. Points out of the whiskers, outliers.