Number of embryos showing “normal” or “mildly or severely ectopic” proliferative cells (pH3+) within the anterior ventral forebrain. n = 15 (not inj. andsnf8MO) and 12 (snf8MO +SNF8WT). Data are expressed as boxplot, for experiments including different batches mean SEM are shown. Number of replicates in (B), one and two (left and right graphs, respectively). In (C) (left), number of replicates: three for not inj,snf8MO,snf8MO +SNF8WT, andsnf8MO +SNF8Y167*;G191D; two forsnf8MO +SNF8P79L;V102I. In (C) (right), number of replicates: six (not inj,snf8MO, andsnf8MO +SNF8WT); three (the other two mutants). Two-sided chi-square test is used to assess statistical significance in a 2x2 contingency table. In (B), not inj. vs. snf8MO,***p = 0.0008 left and****p < 0.0001 right. In (C) (left), not inj. vs.snf8MO,***p = 0.0005;snf8MO vs.snf8MO +SNF8WT,*p = 0.0312;snf8MO +SNF8WTvs. +SNF8Y167*;G191D,**p = 0.0086;snf8MO +SNF8WTvs.snf8MO +SNF8P79L;V102I, not significant (ns, p = 0.06);snf8MO vs. mutants, ns. In (C) (right): not inj. vs.MO,****p < 0.0001;snf8MO vs. snf8MO +SNF8WT,***p = 0.0008;snf8MO +SNF8WTvs. snf8MO +SNF8V102I,**p = 0.0021;snf8MO +SNF8WTvs. snf8MO +SNF8Y167*,**p = 0.0022 (ns: not significant). In (G) non-parametric Kruskal-Wallis test with Dunn’spost hoctest is used for statistical assessment (not inj. vs. snf8MO,*p = 0.0141;snf8MO vs. snf8MO +SNF8WT,**p = 0.0032; ns = not significant). In (H), two-sided chi-square test is used: not inj. vs. snf8MO,*p = 0.0309; not inj. vs. snf8MO +SNF8WT, p = 0.8695 (ns, not significant); andsnf8MO vs. snf8MO +SNF8WT, p = 0.0621 (ns). Fb, forebrain; oe, olfactory epithelium; OC, optic chiasm indicated by a white arrow. Asterisks indicate the eyes.
