Immunofluorescence of organoids generated from PAX8-dCas9-KRAB iPSCs and harvested at day 14, either with no treatment (control, top) or following Dox treatment from day 4 (1 M Dox, bottom), showing GFP (green), PAX8 (magenta), E-cadherin/CDH1 (yellow) with DAPI as counterstain (blue, nuclei). White brackets indicate PAX8+CDH1+cells confined to a GFP−region. Scale bars, 25 m.
