Rescue of MET in cells with PAX8-dCas9-KRAB-mediated PAX8 knockdown by lentiviral overexpression of PAX8. Immunofluorescence of organoids generated from PAX8-dCas9-KRAB iPSCs and harvested at day 14, following Dox treatment from day 4 (1 M Dox, bottom), and either infected with an empty lentivirus (EF1::ctrl/mCherry, top) or PAX8-overexpressing lentivirus (EF1::PAX8/mCherry, bottom). GFP (green), labels PAX8-knockdown cells, magenta labels mCherry in lentivirus-infected cells (PAX8), and E-cadherin/CDH1 is in yellow. The white box indicates regions magnified in subsequent panels, the white dotted lines mark the outline of a polarized epithelial structure. Scale bars, 20 or 30 m as indicated.
