E13.5 chimeric embryo generated by injection of PGCLCs at 8-cell stage, shown with non-chimera embryo (left). A very low contribution chimera with a few cells near the eye and the forming ear is also shown (right side). PGCLCs were isolated using BVSC double-reporter cell line as perFigure S4B and can be identified by constitutive expression of mCherry. Scale bars, 5 mm.
