Workflow from Scientific Research
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Schematic of derivation of embryonic stem cells (ESCs) from chimeric embryos. PGCs are isolated from E8.5E9.5 embryos by FACS and injected into host embryos at 8-cell stage. After 48 h of in vitro culture, trophectoderm cells are removed by immunosurgery, and primary colonies are then cultured in 2i/LIF, then expanded by passaging. PGC-ESCs and host-ESCs are subsequently separated by FACS. Created with BioRender.com.
#Workflow#Illustration#Embryonic Stem Cells#Chimeric Embryos#PGCs#FACS#Host Embryos#Trophectoderm Cells#Immunosurgery#Primary Colonies#2i/LIF#PGC-ESCs#Host-ESCs
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