RNAi knockdown of Sox21b in D. melanogaster. Genotypes in bold represent Sox21b RNAi knockdown lines. KD, knockdown. UAS lines indicate the parental control lines with only the UAS-RNAi. Full genotypes for the lines used can be found in the method details. Asterisks above the knockdown line indicate the levels of statistical significance between the RNAi knockdown and associated parental GAL4 driver: orange for NP6333-GAL4 and pink for POXN-GAL4. The lines below, adjoining the RNAi knockdown to the parental UAS-RNAi line, indicate the level of statistical significance between each pair. ***p < 0.001 and **p < 0.01. Within each violin plot, the following values are represented: the median as the bold horizontal line, the box as the interquartile range, and the range as the vertical line. n > 13 for each line.
