Bar Plot from Scientific Research

Mosaic wing discs with clones (cyan) expressing either UAS-robo3.HA (Robo3OE) (A) or UAS-robo3-RNAi (Robo3RNAi) (B). TRE-RFP expression is reporting JNK pathway activity (gray or magenta in all panels). Antibody staining against the cleaved effector caspase (Dcp1) visualizes apoptosis (yellow). Phalloidin visualizes cortical F-actin (gray). White frames in (A) and (B) mark the region shown in (A′) and (B′). White frames in (A′) and (B′) mark the regions shown in (A″) and (B″). Image sets are shown at the same scale. Please note the actin enrichment at clone interfaces in (A″) (green arrows). (A‴) Graphs depicting mean fluorescence intensity of TRE-RFP reporter and mean Dcp1 intensity in the zones of measurement around clones, as depicted inFigure 3F. Graphs display results for mosaic discs containing UAS-Robo3.HA (Robo3OE)-expressing clones. See Figure S5C‴ for UAS-GFP-labeled clonal cells as wild-type control. Sample number (n) for individual wing discs and p values of a repeated measured one-way ANOVA with Tukey’s multiple comparisons test are displayed in graphs. Error bars represent mean and 95% confidence interval.

DOI