Mosaic wing discs with aberrant expression of cell surface molecules. Clones express GFP (cyan) and were exposed to either ectopic expression (OE) of UAS constructs or downregulation of expression by UAS-RNAi (RNAi) constructs for individual cell surface molecules, as indicated in each panel. White frame marks regions shown in (D′). Images in (A)–(N) are shown at the same scale. JNK responses in (A)–(C) have been observed in n ≥ 3 experimental replicates for this study and previous studies.4JNK responses in (F), (H), and (J)–(L) have been observed in n ≥ 3 experimental replicates for this study. JNK response in (G), (I), (M), and (N) has been observed in n ≥ 3 wing discs in one experimental replicate, which corresponds to the initial candidate screen (seeFigure S1F). Related to Figure S1. TRE-RFP expression is reporting JNK pathway activity (gray or magenta in all panels).
