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Schematic of the postmortem localization procedure. Left: micro-CT scans in the horizontal plane in two different example axial locations with manually placed markers (red dots) to identify the position of the midsagittal plane for proper orientation. Middle: micro-CT scan in the sagittal plane with some example manually placed markers for identified anatomical markers to enable registration to the Allen Mouse Brain Common Coordinate Framework atlas32(bottom). Right: micro-CT scans in the horizontal plane of the grid surface (top) and coronal plane (bottom) showing the resulting automated tracing of 4 optical fibers.

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