Quantification of pPDH intensity ratio between WD and control groups in brain regions exhibiting increased FOS expression following water deprivation. Brain regions identified as having upregulated FOS expression from (D) were filtered to exclude regions associated with the blood-brain barrier or regions that were too small for accurate assessment (gray label in D). FOS fold change is color-coded on the left. Control: n = 8; WD: n = 7. Error bars are mean SEM. Statistics determined by multiple two-sided t tests corrected for multiple comparisons with the two-stage step-up (Benjamini, Krieger, and Yekutieli).*False discovery rate (FDR) < 10%.
