Ripley H-function analysis of docked vesicle distribution in individual AZs revealed that the null model was rejected (maximum difference test) for 13.3% of P7–9, 22.2% of P14–16, and 60.0% of P21–23 AZs. Data in (C)–(F) and (I) were obtained from chemically fixed tissue; data in (G), (H), (J), and (K)–(M) were obtained from cryofixed tissue. See alsoFigure S6andTables S2andS3.