RORγt+ helios- percentages out of FOXP3+ cells in colon. Single cells were isolated from colon lamina propria. Immune cells were analyzed by flow cytometry. Horizontal lines represent the means. Each dot represents a mouse. Green, GF mice; blue, mice monocolonized with B. fragilis; yellow, mice monocolonized with B. fragilis and Barc2635. ***p < 0.001, ****p < 0.0001, one-way analysis of variance (ANOVA). See also Figure S5.
