Normalized Capture-C counts usingscyl(left) orchrb(right) promoters as baits, indicated by vertical-colored lines (magenta, cyan). Capture-C shown for wild-type embryos (control), embryos with deletion of thescylgene (second track), middle loop anchor (LA) (third track), andscylLA (lower track). Zoom-in (underneath) indicates the location of deletions (DEL, black) relative to the genes (blue) and the Capture-C probes (magenta/cyan) at the three LAs. The location of two previously identified enhancers (CRMs,cisregulatory modules) at the middle LA is indicated (green). Deletion of thescylLA abrogates the outer loop (lower track, arrow).
