Local map of a JetD dimer bound to plasmid DNA (Ai) (seeTable 1,Figures S5andS8, andSTAR Methods; overall resolution: 6.34 Å) and AlphaFold2-predicted models of JetD domains and B-form DNA fitted into the map (Aii). The interface between JetD and JetC is marked by the box and shown in larger magnification in (B) The positions of the putative active site residue mutated in JetD (E248A, one of three residues coordinating Mg2+ions in TOPRIM domains) are indicated in sphere representation in dark red colors. Notably, extra density not unambiguously assigned was marked by asterisks, which may correspond to a short JetB amino-terminal helix that was predicted to bind to JetD aCAP9(*), while the remaining may originate from neighboring JetB sequences (**).
