Left: plasmid restriction by mutant JET. Graph showing the percentage of plasmid (pBAD)-containingE. colicells in a cell population grown for ten generations without antibiotic selection with or without JET induction by arabinose addition. Means, standard deviations, and individual data points from three independent experiments are shown. Right: model of the JetD(aCAP)-JetB(larynx) interface depicting residues tested by mutagenesis are stick representation (JetABCD*core model,Figure 4B). The JetC protein surface is displayed in semi-transparent blue colors.
