Spatial distribution heatmaps of NusG tracks with a categorization threshold of D*= 0.31 for 311 cells with lengths of ~2–3 m, having ~2 nucleoids. Top: a heatmap for molecules with D*< 0.31 m²/s, representing mainly the VS-NusG species (82% of all tracks). Bottom: a heatmap for molecules with D*> 0.31 m²/s, representing the F-NusG species (99% of all tracks).