(Left) Insertion into mitochondria isolated from WT K562 cells (Figure S8E) of endogenous substrates as assessed by proteinase K digestion (PK) of CISD1 (SA), TOMM70 (SA), OMP25 (TA), and MAVS (TA) in the presence of matched concentrations of WT TTC1 or the I271D mutant (5 μM). (Right) Quantification of substrate insertion (seeFigure S8F for totals). p-values in the order of buffer versus WT, buffer versus I271D, and WT versus I271D for i) CISD1: 0.0169, 0.0677, 0.0044; ii) TOMM70: 0.0038, 0.8442, 0.0052; iii) OMP25: 0.0144, 0.0663, 0.0086; and iv) MAVS: 0.0054, 0.6315, 0.0007. SeeFigures S7andS8.
