Gel from Scientific Research
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CISD1 was translatedin vitroin the presence of35S in the PURE system with either no chaperone or the indicated purified proteins (WT and I271D TTC1, calmodulin). The translations were run on a sucrose gradient and the extent of the substrate remaining soluble was determined by autoradiography.
#Gel#CISD1#in vitro Translation#PURE System#Chaperone#Purified Proteins#TTC1#Calmodulin#Sucrose Gradient#Autoradiography
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