Immunofluorescence microscopy imaging of thymic sections at steady state and days 1, 4, and 7 after injury, stained for Foxp3-expressing Treg cells (red), CD31-expressing endothelium (orange), cytokeratin (CK)14-expressing regions (white), and DAPI (blue). M, medulla; C, cortex. Regeneration at day 7 after injury in 1- to 2-month-old B6.Foxp3DTRthymi from littermate mice treated with either 20 ng/g DT or PBS i.p. on day -1 and 3 h prior to SL-TBI:
