ATAC-seq was performed with wild-type and Irf3-/- BMDMs left unstimulated or stimulated for 120 min in the presence of CHX to prevent secondary response proteins from masking IRF3 requirements during the primary response. Induced ATAC-seq signals (RPKM) from wild-type and mutant cells were compared by scatter plot (top) and volcano plot (bottom) for the 40 promoters (left) and 1,494 intergenic (right) ATAC-seq peaks induced >5-fold. Peaks overlapping with a strong (peak score > 90) IRF3 ChIP-seq peak (red) are distinguished from peaks with weak (peak score < 90) IRF3 peaks (blue) and from peaks lacking IRF3 peaks (black).
