ATAC-seq was performed with a wild-type macrophage line and two clonal Rela-/-Rel-/- lines. 1,001 primary response ATAC-seq peaks induced >5-fold in the wild-type line were separated into ten bins based on statistical significance (-log[p value]) of maximum induction at the time point at which maximum induction was achieved in two independent experiments. Scatterplots are shown for the four bins with the strongest statistical significance ranges. The scatterplots show ATAC-seq RPKM from wild-type (x axis) and Rela-/-Rel-/- (y axis) cells at the time point that yields the maximum difference (p value). The solid diagonal line represents equal RPKM and the dashed line, average RPKM in the mutant lines that is 33% of the wild-type RPKM. Peaks that are >33% of wild type in the mutant lines are colored in red. Numbered peaks (1-5) are shown in (B).
