ATAC-seq was performed with lipid-A-stimulated macrophages (0, 30, 60, and 120 min) in the absence and presence of CHX. Peaks induced >5-fold were separated into eight bins based on sensitivity of peak induction to CHX, with motif enrichment determined as in (A). Bins 1 and 8 contain peaks with the greatest (secondary response) and least (primary response) CHX sensitivity, respectively. Arbitrary separations between the primary and secondary responses are shown.
