Microscopy from Scientific Research

Representative confocal images of DHX9 and HNRNPM or HuR in WT and G3K HeLa cells 8 h after 4sU + UVA stress. Auxin was added immediately after 4sU + UVA stress. HuR is a universal marker for all SG subtypes, while HNRNPM is a DHX9 SG-specific marker identified by FANCI-DHX9-SG proteomics. Scale bars, top, 5 μm and bottom, 2 μm. G3K: auxin-treatedG3BP1−/−; G3BP2-AID; Myc-Tir1HeLa, seeFigure S8B. Images were saturated to display the cytosolic retention of indicated proteins.

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