Scatter Plot from Scientific Research

A UMAP of 1590 features is created and clustered (i), yielding three populations of synapses. The individual synapses are labeled with their identification number from the analysis workflow and are highlighted in the UMAP. The top left shows the multiplexed overlay of excitatory and inhibitory markers alongside ac-Tubulin (dark green background) as cytoskeleton reference. The unique composition of pre- and postsynaptic markers (Bassoon, Homer1, Gephyrin), as well as the neurotransmitter transporter (VGlut1, VGAT) allows us to label the UMAP clusters as excitatory (blue), inhibitory (purple) and a distinct mixed synaptic population (yellow). (ii–vi) highlights the normalized protein content (grayscale) of a single pre- or postsynaptic marker in the UMAP. The top left shows a single channel image of the same region for each protein.

DOI