Bright-field and re-scan confocal images of hnRNPA1A condensates in the presence of 50 ng l –1 polyU labelled with Alexa Fluor 647 Hydrazide. This condition was taken as representative of the second regime, colour-coded with blue in Fig. 3 . Already after 30 min, we observe a higher ThT signal at the interface of heterotypic condensates. In this condition, condensates dissolve completely or partially, and within a few hours are replaced by amyloid fibrils. Imaging of hnRNPA1A aggregation in the presence of labelled polyU was performed with two distinct protein preparations. The time of condensate dissolution varied by a few hours depending on the protein batch.
